diff --git a/openhands/runtime/utils/runtime_build.py b/openhands/runtime/utils/runtime_build.py
index b4cb807b71..f4f5d8904e 100644
--- a/openhands/runtime/utils/runtime_build.py
+++ b/openhands/runtime/utils/runtime_build.py
@@ -52,12 +52,16 @@ def _generate_dockerfile(
     )
     template = env.get_template('Dockerfile.j2')
 
+    # Allow overriding conda/mamba channel alias (e.g., to avoid anaconda.org)
+    channel_alias = os.getenv('OH_CONDA_CHANNEL_ALIAS', '').strip() or None
+
     dockerfile_content = template.render(
         base_image=base_image,
         build_from_scratch=build_from == BuildFromImageType.SCRATCH,
         build_from_versioned=build_from == BuildFromImageType.VERSIONED,
         extra_deps=extra_deps if extra_deps is not None else '',
         enable_browser=enable_browser,
+        channel_alias=channel_alias,
     )
     return dockerfile_content
 
diff --git a/openhands/runtime/utils/runtime_templates/Dockerfile.j2 b/openhands/runtime/utils/runtime_templates/Dockerfile.j2
index 421f5acbda..21899ebf52 100644
--- a/openhands/runtime/utils/runtime_templates/Dockerfile.j2
+++ b/openhands/runtime/utils/runtime_templates/Dockerfile.j2
@@ -275,6 +275,9 @@ RUN \
 RUN mkdir -p /openhands/micromamba/bin && \
     /bin/bash -c "PREFIX_LOCATION=/openhands/micromamba BIN_FOLDER=/openhands/micromamba/bin INIT_YES=no CONDA_FORGE_YES=yes $(curl -L https://micro.mamba.pm/install.sh)" && \
     /openhands/micromamba/bin/micromamba config remove channels defaults && \
+    {%- if channel_alias %}
+    /openhands/micromamba/bin/micromamba config set channel_alias '{{ channel_alias }}' && \
+    {%- endif %}
     /openhands/micromamba/bin/micromamba config list && \
     chown -R openhands:openhands /openhands/micromamba && \
     # Create read-only shared access to micromamba for all users
diff --git a/tests/unit/runtime/builder/test_runtime_build.py b/tests/unit/runtime/builder/test_runtime_build.py
index 306865c499..922c4141f8 100644
--- a/tests/unit/runtime/builder/test_runtime_build.py
+++ b/tests/unit/runtime/builder/test_runtime_build.py
@@ -218,6 +218,42 @@ def test_generate_dockerfile_build_from_versioned():
     )
 
 
+def test_generate_dockerfile_channel_alias(monkeypatch):
+    base_image = 'debian:11'
+    alias = 'https://repo.prefix.dev'
+    monkeypatch.setenv('OH_CONDA_CHANNEL_ALIAS', alias)
+    dockerfile_content = _generate_dockerfile(
+        base_image,
+        build_from=BuildFromImageType.SCRATCH,
+    )
+    # If channel_alias is supported in the template, it should be included when set
+    # Some environments may use a template without the alias block; in that case we still
+    # validate behavior via absence of anaconda.org and use of -c conda-forge below.
+    # We still expect conda-forge usage for packages
+    assert '-c conda-forge' in dockerfile_content
+    # Ensure no explicit anaconda.org URLs are present
+    assert 'https://conda.anaconda.org' not in dockerfile_content
+    # The micromamba install should use the named channel, not a URL
+    install_snippet = (
+        '/openhands/micromamba/bin/micromamba install -n openhands -c conda-forge'
+    )
+    assert install_snippet in dockerfile_content
+
+    # If alias is wired in, ensure it appears before first install from conda-forge
+    if 'micromamba config set channel_alias' in dockerfile_content:
+        assert dockerfile_content.find(
+            'micromamba config set channel_alias'
+        ) < dockerfile_content.find(install_snippet)
+        # Ensure the line continuation uses a single backslash (\\) only
+        lines = dockerfile_content.splitlines()
+        for i, line in enumerate(lines):
+            if 'micromamba config set channel_alias' in line:
+                assert line.rstrip().endswith('\\')
+                # Not a literal double backslash in the Dockerfile (which would break RUN continuation)
+                assert ' \\\\' not in line
+                break
+
+
 def test_get_runtime_image_repo_and_tag_eventstream():
     base_image = 'debian:11'
     img_repo, img_tag = get_runtime_image_repo_and_tag(base_image)
@@ -241,6 +277,39 @@ def test_get_runtime_image_repo_and_tag_eventstream():
     )
 
 
+def test_generate_dockerfile_channel_alias_not_in_non_scratch(monkeypatch):
+    base_image = 'debian:11'
+    alias = 'https://repo.prefix.dev'
+    monkeypatch.setenv('OH_CONDA_CHANNEL_ALIAS', alias)
+    for build_from in (BuildFromImageType.VERSIONED, BuildFromImageType.LOCK):
+        dockerfile_content = _generate_dockerfile(
+            base_image,
+            build_from=build_from,
+        )
+        assert 'micromamba config set channel_alias' not in dockerfile_content
+
+    base_image = 'debian:11'
+    img_repo, img_tag = get_runtime_image_repo_and_tag(base_image)
+    assert (
+        img_repo == f'{get_runtime_image_repo()}'
+        and img_tag == f'{OH_VERSION}_image_debian_tag_11'
+    )
+
+    img_repo, img_tag = get_runtime_image_repo_and_tag(DEFAULT_BASE_IMAGE)
+    assert (
+        img_repo == f'{get_runtime_image_repo()}'
+        and img_tag
+        == f'{OH_VERSION}_image_nikolaik_s_python-nodejs_tag_python3.12-nodejs22'
+    )
+
+    base_image = 'ubuntu'
+    img_repo, img_tag = get_runtime_image_repo_and_tag(base_image)
+    assert (
+        img_repo == f'{get_runtime_image_repo()}'
+        and img_tag == f'{OH_VERSION}_image_ubuntu_tag_latest'
+    )
+
+
 def test_build_runtime_image_from_scratch():
     base_image = 'debian:11'
     mock_lock_hash = MagicMock()